Era ADVANCED ANALYTICS

Erika Amstalden van Hove

Mass Spectrometry, MS imaging, LC methods, sample preparation, method development and research

Services provided by ERA ADVANCED ANALYTICS

Analytical Chemistry Area

Mass Spectrometry, MS imaging, LC methods, sample preparation, method development and research

The goal of ERA ADVANCED ANALYTICS is to provide insight in several types of analytical methods to other researchers and improve research quality and reach. This can be achieved through courses, on-site activities using their own instruments, help to set-up mass spectrometry based laboratory and knowledge exchange on mass spectrometry application (e.g. MS Imaging) on different research areas.

Advice

I can help your research development with advise about several sample preparation techniques, such as MS, MS imaging, LC methods, sample preparation and method development, as well as the adequate instrumentation to achieve specific goals.

Research

The development of new research paths is by no means trivial. We constantly need do think "out of the box" in order to come up with new research ideas, that can be both exciting and useful. In order to do it we also need funding. Together, we can write and develop strong research plans and grant proposals.

Training

General courses on mass spectrometry, mass spectrometry imaging, proteomics and others

Courses tailored to your specific needs

Training on site with your instruments, adapting well known methods to your needs and your laboratory.

Preparation of training material including translations if necessary.

Publications can be downloaded at www.researchgate.net or upon request.

Why should we partner

  • The success of MS based research relies heavily in the sample preparation step and in the choice right methodology and instrumentation. Although the sample preparation for MS based experiments may be well documented, changes and adaptations are often necessary for an optimal result.
  • The right changes in well know sample preparation, development of new methodology and the choice of right instruments requires expertise, and can be time demanding and costly in the absence of an expert. I can provide the expertise needed in these cases, as well as shown how to make the most of the instrumentation already available in you laboratory. This results in enormous amount of time saving, and currently in cost decrease as well.
  • A well trained personnel is essential for the continuance of high level research, and therefore specific trainings can also be provided on site.

About Erika

A strong driver for me are the challenges and promises of applying mass spectrometry and other analytical techniques to unlock new insights into the role of different substances, from single elements to macromolecules. I have a broad experience in analytical chemistry, in particular in mass spectrometry (MS), MS imaging, proteomics, several LC methods and Surface Plasmon Resonance(SPR). I have also worked with targeted analysis, label-free methods, several ionization methods (e.g. ESI, MALDI, LDI) as well as compounds synthesis, purification, qualitative and quantitative analysis. My research have always been driven by improvement in life quality, since my time as bachelor student, and has included research on Alzheimer disease, breast cancer and drug detection, to cite a few.

Being able to perform sensitive and quantitative analysis of molecules, and thus help unravel disease pathways and improve treatment options is something that speaks directly to my heart. Additionally, the application of knowledge to solve problems and create new research in different fields is always a welcome challenge and as proved to be very fruitful in several cases.

about-image

Amstalden van Hove, E.R., New methods for multimodal imaging MS of histological tissue sections, PhD thesis (2011)

Amstalden van Hove, E.R. et al., A Concise Review of Mass Spectrometry Imaging. Journal of Chromatography A 1217 (25), 3946-3954 (2010) 

Amstalden van Hove, E.R. et al.,  Multimodal Mass Spectrometric Imaging of Small Molecules Reveals Distinct Spatio-Molecular Signatures in Differentially Metastatic Breast Tumor Models, Cancer Research 70 (22), 9012-9021 (2010) 

Amstalden van Hove, E.R. et al., An alternative paper based tissue washing method for Mass Spectrometry Imaging: Localized Washing and Fragile Tissue Analysis, Journal of The American Society for Mass Spectrometry,  22 (10),1885-90 (2011)

Amstalden van Hove, E.R. et al., Factors affecting detection on quantitative studies of pharmaceutical compounds by MSI, Thesis chapter.

Jiang, L. et al, Combined MR, fluorescence and histology imaging strategy in a human breast tumor xenograft model. NMR in Biomedicine 26, 285-298 (2013).

Fornai, L. et al, Three-dimensional molecular reconstruction of rat heart with mass spectrometry imaging. Analytical and Bioanalytical Chemistry 404, 2927-2938 (2012).

Chughtai, K. et al., Fiducial markers for combined 3-dimensional mass spectrometric and optical tissue imaging. Analytical Chemistry 84 (4):1817-23 (2012).

Hanselmann, M. et al., Toward Digital Staining using Imaging Mass Spectrometry and Random Forests. Journal of Proteome Research 8 (7), 3558 (2009).

Hanselmann, M. et al., Concise Representation of Mass Spectrometry Images by Probabilistic Latent Semantic Analysis. Analytical Chemistry 80 (24), 9649 (2008).

Klinkert, I. et al., Tools and strategies for visualization of large image data sets in high-resolution imaging mass spectrometry. Review of Scientific Instruments 78 (5) (2007).

Heeren, R. M. A. et al., High Resolution Mass Spectrometric Imaging of Cells and Tissue: MALDI and Surface Enhanced SIMS Put to Work. Microscopy and Microanalysis 12, 1210 – 1211(2006).

Heeren, R. M. A. et al., Why don't biologists use SIMS? A critical evaluation of imaging MS. Applied Surface Science 252 (19), 6827 (2006).

Luxembourg, S. L. et al., The molecular scanner in microscope mode. Rapid Communications in Mass Spectrometry 20 (22), 3435 (2006).

Przybylski, M et al., Identification of antibody-paratope peptides by affinity-proteomics using high resolution FT-ICR mass spectrometry. Biopolymers 80 (4), 603 (2005).

Stefanescu, R. et al., Mass spectrometric approaches for elucidation of antigen-anti body recognition structures in molecular immunology. European Journal of Mass Spectrometry 13 (1), 69 (2007).

Przybylski, M. et al., Affinity Proteomics and Mass Spectrometric epitope analysis: powerfull tools for identification of vaccine lead structures against Alzheimer's disease. Journal of Peptide Science 10, 294 (2004).

Przybylski, M. et al., Affinity- and antibody-proteomics using high resolution FTICR-MS: powerful tools for elucidation of vaccine lead structures and molecular diagnostics. Molecular & Cellular Proteomics 3 (10), S45 (2004).

NVMS 2010
Amstalden van Hove, E.R., Dillen, L., De Man, H., Anciaux, K., Cuyckens, F., Heeren, R.M.A., Characterization of ion suppression and quantitative analysis of drug distribution on tissue by mass spectrometry imaging, The Dutch Society for Mass Spectrometry fall meeting, Amsterdam, The Netherlands.

ASMS 2010
Amstalden van Hove, E.R., Smith, D., Dillen, L., De Man, H., Anciaux, K., Cuyckens, F., Heeren, R.M.A.,SIMS and MALDI Mass Spectrometry Imaging of Drugs and Metabolites in Whole-Body Mouse Sections, 58th ASMS Conference on Mass Spectrometry and Allied Topics, Salt Lake City, Utah, USA.

ASMS 2009
Amstalden van Hove, E.R., Klinkert, I., Greenwood, T. R., Czornak, K., Heeren, R.M.A., Glunde, K (2009) 3-dimensional reconstruction of breast tumor xenografts with combined Mass Spectrometry Imaging and Magnetic Resonance Spectroscopic Imaging. 57th ASMS Conference on Mass Spectrometry and Allied Topics, Philadelphia, Pennsylvania, USA.

ASMS 2009 (co-author) (Michael Hanselmann)
Hanselmann, M., Köthe, U., Kirchner, M., Renard, B. Y., Amstalden van Hove, E.R., Glunde, K., Heeren, R.M.A.,  Hamprecht, F.A. (2009) Automated Classification and Grading of Tumors in Mass Spectrometric Images using postprocessed Random Forests. 57th ASMS Conference on Mass Spectrometry and Allied Topics, Philadelphia, Pennsylvania, USA.

IMSC 2009
Amstalden van Hove, E.R., Czornak, K., Fornai, L., MacAleese, L., Greenwood, T.R., Glunde, K., Heeren, R.M.A. (2009)PaperBlot, an Innovative Sample Washing Protocol for Imaging Mass Spectrometry of Biological Tissue Sample. 18th International Mass Spectrometry Conference, Bremen, Germany.

NPC 2008
Amstalden van Hove, E.R., Klinkert, I., Greenwood, T. R. Glunde, K., Heeren, R.M.A. (2008) Small and big molecules: a combined MS Imaging experiment. Netherlands Proteomics Centre Progress MeetingUtrecht, The Netherlands.

ASMS 2008
Amstalden van Hove, E.R., Klinkert, I., Greenwood, T. R. Glunde, K., Heeren, R.M.A. (2008) From lipids to proteins in one single tissue. 56th ASMS Conference on Mass Spectrometry and Allied Topics, Denver, Colorado, USA.

ASMS 2008 (co-author)
MacAleese, L., Amstalden van Hove, E.R., Stauber, J., Heeren, R.M.A. (2008) Imaging Mass Spectrometry clocking in with Proteomics: on-tissue MS/MS for proteins identification. 56th ASMS Conference on Mass Spectrometry and Allied Topics, Denver, Colorado, USA.

HUPO 2008
Amstalden van Hove, E.R., Klinkert, I., Greenwood, T. R. Glunde, K., Heeren, R.M.A. (2008) From lipids to proteins in one single tissue. HUPO 7th Annual World Congress 2008, Amsterdam, The Netherlands.

Sanibel 2007
Amstalden van Hove, E.R., Klinkert, I., Greenwood, T. R. Glunde, K., Heeren, R.M.A. (2007). Imaging-MS for identification of biomarkers in xenograft breast cancer tissue. 18th Sanibel Conference on Mass Spectrometry: Imaging Mass Spectrometry. Sanibel Island, Florida, USA.

Amstalden v. Hove, E.R., Bon, E., Luider, T.M., Steegers, E.A.P., Steegers, R., Ursem, N., Heeren, R.M.A. (2007) A study of heart development in chicken embryos using SIMS-Imaging. 18th Sanibel Conference on Mass Spectrometry: Imaging Mass Spectrometry, Sanibel Island, Florida, USA.

ASMS 2007 (co-author)
Heeren, R.M.A., Amstalden van Hove, E.R., Froesch, M., Klinkert, I., Greenwood, T. R. Glunde, K. (2007) Imaging Mass Spectrometry of Breast Cancer tissue : Application and new developments. 55th ASMS Conference on Mass Spectrometry and Allied Topics, Indianapolis, Indiana, USA.

NPC 2007
Amstalden v. Hove, E.R., Bon, E., Luider, T.M., Steegers, E.A.P., Steegers, R., Ursem, N., Heeren, R.M.A. (2007) A study of heart development in chicken embryos using SIMS-Imaging. Netherlands Proteomics Centre Progress Meeting Utrecht, The Netherlands.

Biomarker Symposium 2006
Amstalden van Hove, E.R., Luxembourg, S.L, Piersma, S., Glunde, K., Sobczak-Elbourne, I., Van der Zee, A.G.J., Bischoff, R., Vaezzadeh, A.R., Zimmermann-Ivol, C.G., Hochstrasser, D., Heeren, R.M.A. (2006) Imaging-MS as a tool for biomarker identification in tissue sections. Biomarker Symposium, Amsterdam, The Netherlands.

ASMS 2006
Amstalden van Hove, E.R., Luxembourg, S.L, Piersma, S., Glunde, K., Sobczak-Elbourne, I., Bischoff, R., Vaezzadeh, A.R., Zimmermann-Ivol, C.G., Heeren, R.M.A. (2006) Imaging-MS for identification of biomarkers in tissue sections. 54th ASMS Conference on Mass Spectrometry and Allied Topics, Seattle, Washington, USA.

IMSC 2006
Amstalden van Hove, E.R., Luxembourg, S.L, Piersma, S., Glunde, K., Sobczak-Elbourne, I., Bischoff, R., Vaezzadeh, A.R., Zimmermann-Ivol, C.G., Heeren, R.M.A. (2006) Imaging-MS for identification of biomarkers in tissue sections. 17th International Mass Spectrometry Conference, Prague, Czech Republic.

ASMS 2005 (co-author)
Römpp, A., Konijnenburg, M., Taban, I., Duursma, M.C., Amstalden, E.R., Thieu, V.A., Kirsch, D., Luider, T., Spengler, B., Heeren, R.M.A. (2005) Strategies for the efficient use of Fourier Transform Ion Cyclotron Mass Spectrometry. 53rd ASMS Conference on Mass Spectrometry and Allied Topics, San Antonio, Texas, USA.

DGMS 2004
Amstalden, E.R., Marquardt, A., Youhnovski, N., Damoc, E., Przybylski, M. (2004) Antibody proteomics: Elucidation of paratope structure recognising a ß-amyloid specific epitope using high resolution FT-ICR mass spectrometry. 6th Conference of the German Society of Mass Spectrometry (DGMS). Leipzig, Germany.

EFTMS 2004
Amstalden, E.R., Marquardt, A., Youhnovski, N., Damoc, E., Przybylski, M. (2004) Antibody proteomics: Elucidation of paratope structure recognising a ß-amyloid specific epitope using high resolution FT-ICR mass spectrometry. 7th European Workshop on Fouriertransform Mass Spectrometry. Konstanz, Germany. (internal poster price)

ASMS 2004 (co-author)
Przybylski, M., Tian, X., Cecal, R., Manea, M., Amstalden, E.R., Hudecz, F.,  McLaurin, J., St.George-Hyslop, P. (2004) Affinity-Proteomics and Mass Spectrometric Epitope Mapping: Powerful Tools for Identification of ß-Amyloid-derived Vaccine Lead Structures against Alzheimer's Disease. 52nd ASMS Conference on Mass Spectrometry and Allied Topics, Nashville, Tennessee, USA.

NVMS (2004)
Amstalden, E.R., Marquardt, A., Youhnovski, N., Damoc, E., Przybylski, M. (2004) Antibody proteomics: Elucidation of paratope structure recognising a ß-amyloid specific epitope using high resolution FT-ICR mass spectrometry. The Dutch Society for Mass Spectrometry (NVMS) Meeting, Rolduc, The Netherlands.

Experience

2013 until present

Extra-curricular

Scientific advisor for PhD students regarding sample preparation techniques and methodology development from different fields as well as mass spectrometry related issues.

Development and execution of interior styling-concepts. Focus on materials.

September 2011- April 2013

Postdoctoral

University: VU University Amsterdam, Amsterdam, The Netherlands.

Research Project: Innovative Analytical Methodologies for Biopharmaceuticals. The research included the use of mass spectrometry, spectroscopy and Surface Plasmon Resonance techniques as well as HPLC and LC-MS.

Teaching activities: Master course "Principles of Molecular Clinical Diagnostics II" together with Dr. Henk Lingeman, in 2012. Theoretical and practical lectures for the bachelor course "Biomolecular Screening", with emphasis on proteomics, data analysis and interpretation. Supervision of bachelor students.

April-June 2011

Postdoctoral

University: Universidade Estadual de Campinas, Brazil (Unicamp, State University of Campinas)

Research Project: MALDI mass spectrometry imaging of Parkinson mouse brain and peptidase knockout mouse brain at the Dalton mass spectrometry laboratory, with Prof. Fabio Gozzo.

Teaching activities: Teaching of mass spectrometry imaging techniques from sample preparation to data analysis to master and PhD students as well as a postdoc.

2010

Junior Researcher

Institute: FOM-AMOLF Institute, Amsterdam, The Netherlands in collaboration with Janssen Pharmaceutical Research & Development.

Research Project: Quantitation analysis with mass spectrometry imaging and method development for detection of drug distribution in lyophilized whole body sections from mice and rat with Prof. Dr. Ron M.A. Heeren.

2005 - 2009

PhD

Institute: FOM-AMOLF Institute, Amsterdam, The Netherlands

Research Project: Mass spectrometry imaging of sections from different xenograft human breast tumor types; methodological development (e.g. in-situ tryptic digestion, sample preparation protocols), 3D construction of tumors with mass spectrometry imaging based on molecular distribution with Prof. Dr. Ron M.A. Heeren in collaboration with Kristine Glunde, associate professor at Johns Hopkins University School of Medicine.

Additional: Two weeks of training in the cell culture, tumor inoculation, tumor extraction, sectioning and several types of optical analysis techniques at Johns Hopkins University School of Medicine.

2000 - 2004

Researcher and Master Thesis

University: University of Konstanz, Germany

Courses: Biopolymer & macromolecular analysis, Analytical chemistry, Inorganic chemistry, Physical Chemistry, Organic Chemistry (Aromatic and Heterocyclic compounds)

Research Project: Proteomic analysis, study of an antibody for Alzheimer disease by mass spectrometry (MALDI-TOF and -FTICR), combined with electrophoreses for amino-acid identification; hydrogen-deuterium exchange in combination with ESI-MS and antibody structure modeling based on MS data with Prof. Dr. Dr. h.c. Przybylski.

Master's Thesis: Antibody Sequence Identification through affinity experiments and spectrometry methods, with Prof. Dr. Dr. h.c. Przybylski.

March 1994 - June 2000

Bachelor

University: Universidade Estadual de Campinas, Brazil (Unicamp, State University of Campinas-Top rated university)

Study: Chemistry

Specialization: Inorganic Chemistry

Main courses: physical chemistry (e.g. surfactants and corrosion), biochemistry, organic and inorganic chemistry, physics, and mathematics

Research Project: Synthesis and application of an organometallic compound as catalyst for pollutant gases, such as CO and NO and others provenience of auto motor combustion, with Prof. Dr. E.J.S. Vichi.

       

Languages

Portugese

fluent (native language)

English

fluent

Dutch

fluent

German

fluent

Contacts

ERA ADVANCED ANALYTICS

Phone

+31 6 41361912

Chamber of Commerce registration

KVK 63121492

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